Brightfield multiplex immunohistochemistry with multispectral imaging
نویسندگان
چکیده
منابع مشابه
Stain Unmixing in Brightfield Multiplex Immunohistochemistry Images
Multiplex immunohistochemistry (IHC) staining is a newly emerging technique for the detection of multiple biomarkers within a single tissue section and has become more popular due to its significant efficiency and the rich diagnostic information it contains. Therefore, to accurately unmix the IHC image and differentiate all the stains is of tremendous clinical importance since it is the initial...
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Multiplex immunohistochemistry (IHC) staining is a new, emerging technique for the detection of multiple biomarkers within a single tissue section. The initial key step in multiplex IHC image analysis in digital pathology is of tremendous clinical importance due to its ability to accurately unmix the IHC image and differentiate each of the stains. The technique has become popular due to its sig...
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BACKGROUND Automated scanning devices and image analysis software provide a means to overcome the limitations of manual semiquantitative scoring of immunohistochemistry. Common drawbacks to automated imaging systems include an inability to classify tissue type and an inability to segregate cytoplasmic and nuclear staining. METHODS Immunohistochemistry for the membranous marker α-catenin, the ...
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Multispectral imaging is a powerful tool that extends the capabilities of the human eye. However, multispectral imaging systems generally are expensive and bulky, and multiple exposures are needed. Here, we report the demonstration of a compact multispectral imaging system that uses vertical silicon nanowires to realize a filter array. Multiple filter functions covering visible to near-infrared...
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ژورنال
عنوان ژورنال: Laboratory Investigation
سال: 2020
ISSN: 0023-6837,1530-0307
DOI: 10.1038/s41374-020-0429-0